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REVIEW
Storage of gastrointestinal endoscopes: when is the safe time for re-use?
Revista Brasileira de Enfermagem. 2022;75(3):e20210216
02-02-2022
Resumo
REVIEWStorage of gastrointestinal endoscopes: when is the safe time for re-use?
Revista Brasileira de Enfermagem. 2022;75(3):e20210216
02-02-2022DOI 10.1590/0034-7167-2021-0216
Visualizações0ABSTRACT
Objectives:
to identify the safe storage time for the use of flexible gastrointestinal endoscopes after high-level disinfection, as well as the defining criteria for this time.
Methods:
an integrative literature review was carried out in the Virtual Health Library, PubMed, Scopus, and Web of Science, considering original articles published since 2000.
Results:
eleven articles were selected, whose storage times ranged from 1 to 56 days, with a predominance of one to seven days (73%). Several criteria were used to define this time, predominantly the premise of efficient processing (100%), use of alcohol flush (64%), use of drying cabinets (18%), among others.
Conclusions:
the criteria for determining the storage time did not show a consensus for clinical practice. Expanding the discussion of this theme with the definition of the minimum necessary conditions is of fundamental importance for the reduction of risks and safety of the procedure and the patient.
Palavras-chave: DisinfectionEndoscopesEquipment ContaminationEquipment ReuseGastrointestinalPatient SafetyVer maisVisualizações0
Resumo
REVIEWStorage of gastrointestinal endoscopes: when is the safe time for re-use?
Revista Brasileira de Enfermagem. 2022;75(3):e20210216
02-02-2022DOI 10.1590/0034-7167-2021-0216
Visualizações0ABSTRACT
Objectives:
to identify the safe storage time for the use of flexible gastrointestinal endoscopes after high-level disinfection, as well as the defining criteria for this time.
Methods:
an integrative literature review was carried out in the Virtual Health Library, PubMed, Scopus, and Web of Science, considering original articles published since 2000.
Results:
eleven articles were selected, whose storage times ranged from 1 to 56 days, with a predominance of one to seven days (73%). Several criteria were used to define this time, predominantly the premise of efficient processing (100%), use of alcohol flush (64%), use of drying cabinets (18%), among others.
Conclusions:
the criteria for determining the storage time did not show a consensus for clinical practice. Expanding the discussion of this theme with the definition of the minimum necessary conditions is of fundamental importance for the reduction of risks and safety of the procedure and the patient.
Palavras-chave: DisinfectionEndoscopesEquipment ContaminationEquipment ReuseGastrointestinalPatient SafetyVer mais
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ORIGINAL ARTICLE
Impact of the contamination time by Escherichia coli on biofilm formation in surgical instruments
Revista Brasileira de Enfermagem. 2021;74(3):e20200759
07-14-2021
Resumo
ORIGINAL ARTICLEImpact of the contamination time by Escherichia coli on biofilm formation in surgical instruments
Revista Brasileira de Enfermagem. 2021;74(3):e20200759
07-14-2021DOI 10.1590/0034-7167-2020-0759
Visualizações0Ver maisABSTRACT
Objectives:
to evaluate the microbial load and adherence of Escherichia coli in different areas of the surgical instrument surface exposed to experimental contamination over time.
Methods:
experimental study in which fragments of crile forceps (serrated, rod and rack) were contaminated by immersion in Tryptic Soy Broth, containing 106 CFU/mL of E. coli, for 1, 2, 4, 6, 8, 12 and 24 hours. Microbial load and bacterial adherence were evaluated using microbiological culture and scanning electron microscopy, respectively.
Results:
there was an increase in the microbial load on the surgical instrument, proportional to the contamination interval, ranging from 102 after 1 hour to 105 CFU/cm2 in 24 hours. The presence of exopolysaccharide was detected after two hours of contamination.
Conclusions:
microbial load and adhesion of E. coli increased over time, reaching 105 CFU/cm2 after 24 hours of contamination, starting biofilm formation after two hours.
Visualizações0Resumo
ORIGINAL ARTICLEImpact of the contamination time by Escherichia coli on biofilm formation in surgical instruments
Revista Brasileira de Enfermagem. 2021;74(3):e20200759
07-14-2021DOI 10.1590/0034-7167-2020-0759
Visualizações0Ver maisABSTRACT
Objectives:
to evaluate the microbial load and adherence of Escherichia coli in different areas of the surgical instrument surface exposed to experimental contamination over time.
Methods:
experimental study in which fragments of crile forceps (serrated, rod and rack) were contaminated by immersion in Tryptic Soy Broth, containing 106 CFU/mL of E. coli, for 1, 2, 4, 6, 8, 12 and 24 hours. Microbial load and bacterial adherence were evaluated using microbiological culture and scanning electron microscopy, respectively.
Results:
there was an increase in the microbial load on the surgical instrument, proportional to the contamination interval, ranging from 102 after 1 hour to 105 CFU/cm2 in 24 hours. The presence of exopolysaccharide was detected after two hours of contamination.
Conclusions:
microbial load and adhesion of E. coli increased over time, reaching 105 CFU/cm2 after 24 hours of contamination, starting biofilm formation after two hours.
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REFLECTIVE
Effectiveness of clinical surface cleaning and disinfection: evaluation methods
- Oleci Pereira Frota
, - Adriano Menis Ferreira ,
- Marcelo Alessandro Rigotti ,
- Denise de Andrade ,
- Nájla Moreira Amaral Borges , [ ... ],
- Marcos Antonio Ferreira Júnior
02-10-2020
Resumo
REFLECTIVEEffectiveness of clinical surface cleaning and disinfection: evaluation methods
Revista Brasileira de Enfermagem. 2020;73(1):e20180623
02-10-2020DOI 10.1590/0034-7167-2018-0623
- Oleci Pereira Frota ,
- Adriano Menis Ferreira ,
- Marcelo Alessandro Rigotti ,
- Denise de Andrade ,
- Nájla Moreira Amaral Borges ,
- Marcos Antonio Ferreira Júnior
Visualizações0ABSTRACT
Objective:
To discuss the methods employed to evaluate the effectiveness of clinical surface cleaning and disinfection (C&D).
Method:
This is a theoretical reflection based on scientific studies and the experience of the authors. Knowledge and current gaps, the need for further studies, and practical application of the methods were approached.
Results:
There are four main methods used to evaluate the effectiveness of clinical surface C&D: visual inspection, fluorescent markers, microbiological cultures, and adenosine triphosphate (ATP) bioluminescence. The first two are used to evaluate the process and to predict adherence to protocols by the staff, and the last two are employed to evaluate the results, therefore being the most relevant to assess the risk of infection.
Final considerations:
The ideal method was not found, because all of them showed limitations. There is a need for strategies to optimize the precision of these methods.
Palavras-chave: Cross InfectionDisinfectionEquipment ContaminationHousekeeping, HospitalProcess Assessment (Health Care)Ver maisVisualizações0Resumo
REFLECTIVEEffectiveness of clinical surface cleaning and disinfection: evaluation methods
Revista Brasileira de Enfermagem. 2020;73(1):e20180623
02-10-2020DOI 10.1590/0034-7167-2018-0623
- Oleci Pereira Frota ,
- Adriano Menis Ferreira ,
- Marcelo Alessandro Rigotti ,
- Denise de Andrade ,
- Nájla Moreira Amaral Borges ,
- Marcos Antonio Ferreira Júnior
Visualizações0ABSTRACT
Objective:
To discuss the methods employed to evaluate the effectiveness of clinical surface cleaning and disinfection (C&D).
Method:
This is a theoretical reflection based on scientific studies and the experience of the authors. Knowledge and current gaps, the need for further studies, and practical application of the methods were approached.
Results:
There are four main methods used to evaluate the effectiveness of clinical surface C&D: visual inspection, fluorescent markers, microbiological cultures, and adenosine triphosphate (ATP) bioluminescence. The first two are used to evaluate the process and to predict adherence to protocols by the staff, and the last two are employed to evaluate the results, therefore being the most relevant to assess the risk of infection.
Final considerations:
The ideal method was not found, because all of them showed limitations. There is a need for strategies to optimize the precision of these methods.
Palavras-chave: Cross InfectionDisinfectionEquipment ContaminationHousekeeping, HospitalProcess Assessment (Health Care)Ver mais - Oleci Pereira Frota
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RESEARCH
Efficiency of cleaning and disinfection of surfaces: correlation between assessment methods
Revista Brasileira de Enfermagem. 2017;70(6):1176-1183
01-01-2017
Resumo
RESEARCHEfficiency of cleaning and disinfection of surfaces: correlation between assessment methods
Revista Brasileira de Enfermagem. 2017;70(6):1176-1183
01-01-2017DOI 10.1590/0034-7167-2016-0608
Visualizações0ABSTRACT
Objective:
to assess the correlation among the ATP-bioluminescence assay, visual inspection and microbiological culture in monitoring the efficiency of cleaning and disinfection (C&D) of high-touch clinical surfaces (HTCS) in a walk-in emergency care unit.
Method:
a prospective and comparative study was carried out from March to June 2015, in which five HTCS were sampled before and after C&D by means of the three methods. The HTCS were considered dirty when dust, waste, humidity and stains were detected in visual inspection; when ≥2.5 colony forming units per cm2 were found in culture; when ≥5 relative light units per cm2 were found at the ATP-bioluminescence assay.
Results:
720 analyses were performed, 240 per method. The overall rates of clean surfaces per visual inspection, culture and ATP-bioluminescence assay were 8.3%, 20.8% and 44.2% before C&D, and 92.5%, 50% and 84.2% after C&D, respectively (p<0.001). There were only occasional statistically significant relationships between methods.
Conclusion:
the methods did not present a good correlation, neither quantitative nor qualitatively.
Palavras-chave: Equipment ContaminationHealth Facility EnvironmentHousekeepingInfectious Disease TransmissionNursing AuditVer maisVisualizações0Resumo
RESEARCHEfficiency of cleaning and disinfection of surfaces: correlation between assessment methods
Revista Brasileira de Enfermagem. 2017;70(6):1176-1183
01-01-2017DOI 10.1590/0034-7167-2016-0608
Visualizações0ABSTRACT
Objective:
to assess the correlation among the ATP-bioluminescence assay, visual inspection and microbiological culture in monitoring the efficiency of cleaning and disinfection (C&D) of high-touch clinical surfaces (HTCS) in a walk-in emergency care unit.
Method:
a prospective and comparative study was carried out from March to June 2015, in which five HTCS were sampled before and after C&D by means of the three methods. The HTCS were considered dirty when dust, waste, humidity and stains were detected in visual inspection; when ≥2.5 colony forming units per cm2 were found in culture; when ≥5 relative light units per cm2 were found at the ATP-bioluminescence assay.
Results:
720 analyses were performed, 240 per method. The overall rates of clean surfaces per visual inspection, culture and ATP-bioluminescence assay were 8.3%, 20.8% and 44.2% before C&D, and 92.5%, 50% and 84.2% after C&D, respectively (p<0.001). There were only occasional statistically significant relationships between methods.
Conclusion:
the methods did not present a good correlation, neither quantitative nor qualitatively.
Palavras-chave: Equipment ContaminationHealth Facility EnvironmentHousekeepingInfectious Disease TransmissionNursing AuditVer mais
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